Phenotypic and functional characterization of proangiogenic monocytes

Phenotypic and Functional Comparison between Flask Adherent and Magnetic Activated Cell Sorted Monocytes Derived Dendritic Cells

Background: Generation of an effective dendritic cell (DC) based cancer vaccine depends on appropriate differentiation of monocytes in vitro. Objective: To compare the effects of monocyte separation methods, flask adherence (Flask-DC) and magnetic activated cell sorting (MACS-DC), on phenotypic and functional characteristics of resultant DCs. Methods: DCs from healthy volunteers were generated ...

Phenotypic Characterization of Human Intermediate Monocytes

A full understanding of the immune system requires full characterization of the cells, and its subsets, which play a role in the process of host defense. The expression pattern of cell surface molecules serves as a major criterion for assigning cells into discrete subsets. The availability of monoclonal reagents, specific fluorescent probes and compatible instrumentation over the past years has...

Phenotypic and Functional Characterization

A study of cell surface phenotypes of PBL of myasthenia gravis (MG) patients showed that their T cells had a significantly higher percentage of 4B4+ T cells (the helper/inducer subset) than ageand sex-matched controls. The PBL of MG patients proliferated significantly higher than those of normal subjects (NS) in response to the purified a chain of the acetylcholine receptor (AChR). Anti-AChR an...

Functional and Phenotypic Characterization

Phenotypic and Functional Characterization